Today's Lab Interpritation

Tuesday in lab we inoculate 3 tubes of Thioglycollate broth to determine the oxygen requirements of our unknowns. We inocullate the tubes with E. coli, Clostridium p. and our unknown. We also did a similare exercise with nutrient broth plates using E.C. and C.P. as our controls to compare our unknowns to.

Results:
E. coli: facultative
C.p: strict anaerobic
#30: facultative

Plate Interpritation:
E. coli: facultative
C.p: anaerobe
#30: facultative, with smooth edges and made the medium a slightly darker color.

From the inoculated broth or our working slants we where to make slides for staining and viewing to determine the shape of our unkown as well as the E. coli and Clostridium p.

My slides made from the slant were a little hard to examine. I remade 3 more from the broth but didn't have time to look at them. I did accidently stain all 3 rather than just one. Hopefully I can use these next week.

Microbe Shapes:
E. coli: bacillus
C.p: cocci clusters
#30: Dr. Belcher thought perhaps mine where cocci that formed chains. I couldn't tell becuase my smear was a little thick.

Next week it's gram staining action like you've never seen!